pND003
(Plasmid
#200949)
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Purpose(Empty Backbone) Yeast surface display destination vector for Golden Gate cloning
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200949 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneUnknown
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Backbone manufacturerUnknown
- Backbone size (bp) 7087
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Vector typeYeast Expression ; Yeast Surface Display
- Promoter GAL1
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Selectable markersTRP1
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Tags
/ Fusion Proteins
- AGA2 (N terminal on backbone)
- HA (N terminal on backbone)
- Myc (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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A portion of this plasmid was derived from a plasmid made byTimothy Whitehead Lab
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.04.13.536781v2 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pND003 was a gift from Timothy Whitehead (Addgene plasmid # 200949 ; http://n2t.net/addgene:200949 ; RRID:Addgene_200949) -
For your References section:
Standardizing cassette-based deep mutagenesis by Golden Gate assembly. Daffern N, Francino-Urdaniz IM, Baumer ZT, Whitehead TA. Biotechnol Bioeng. 2024 Jan;121(1):281-290. doi: 10.1002/bit.28564. Epub 2023 Sep 26. 10.1002/bit.28564 PubMed 37750676
Map uploaded by the depositor.
