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Addgene

pCAGGS-MTS(S31A)-EGFP-V5
(Plasmid #200931)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200931 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4859
  • Total vector size (bp) 5707
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Insert Size (bp)
    849
  • Mutation
    S31A in MTS
  • GenBank ID
  • Promoter CAG
  • Tags / Fusion Proteins
    • V5 (C terminal on insert)
    • mitochondrial targeting sequence (MTS) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer GTTCGGCTTCTGGCGTGTGAC
  • 3′ sequencing primer TATGTCCTTCCGAGTGAGAGAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-MTS(S31A)-EGFP-V5 was a gift from Hung-Chun Chang (Addgene plasmid # 200931 ; http://n2t.net/addgene:200931 ; RRID:Addgene_200931)
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