pSL5069 (pDonor(AAVS1),CRISPR_Pse)
(Plasmid
#200904)
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PurposeEncodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200904 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC57
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
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SpeciesPseudoalteromonas sp. S983
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSL5069 (pDonor(AAVS1),CRISPR_Pse) was a gift from Samuel H. Sternberg (Addgene plasmid # 200904 ; http://n2t.net/addgene:200904 ; RRID:Addgene_200904) -
For your References section:
Targeted DNA integration in human cells without double-strand breaks using CRISPR-associated transposases. Lampe GD, King RT, Halpin-Healy TS, Klompe SE, Hogan MI, Vo PLH, Tang S, Chavez A, Sternberg SH. Nat Biotechnol. 2023 Mar 29. doi: 10.1038/s41587-023-01748-1. 10.1038/s41587-023-01748-1 PubMed 36991112
