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Addgene

pSL2622 (pCas7_Vch)
(Plasmid #200894)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200894 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VchCas7
  • Species
    Vibrio cholerae
  • Tag / Fusion Protein
    • NLS (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL2622 (pCas7_Vch) was a gift from Samuel H. Sternberg (Addgene plasmid # 200894 ; http://n2t.net/addgene:200894 ; RRID:Addgene_200894)

  • For your References section:

    Targeted DNA integration in human cells without double-strand breaks using CRISPR-associated transposases. Lampe GD, King RT, Halpin-Healy TS, Klompe SE, Hogan MI, Vo PLH, Tang S, Chavez A, Sternberg SH. Nat Biotechnol. 2023 Mar 29. doi: 10.1038/s41587-023-01748-1. 10.1038/s41587-023-01748-1 PubMed 36991112
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