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Addgene

spike-A192
(Plasmid #200887)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200887 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-25b
  • Backbone size w/o insert (bp) 5476
  • Total vector size (bp) 9034
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    16 hours
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    spike-A192
  • Species
    E. coli
  • Insert Size (bp)
    3558
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    spike-A192 was a gift from Andrew MacKay (Addgene plasmid # 200887 ; http://n2t.net/addgene:200887 ; RRID:Addgene_200887)

  • For your References section:

    Biomimetic SARS-CoV-2 Spike Protein Nanoparticles. Phan A, Avila H, MacKay JA. Biomacromolecules. 2023 Mar 31. doi: 10.1021/acs.biomac.2c01465. 10.1021/acs.biomac.2c01465 PubMed 37001147
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