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pNIC28-HiBIT-WDR5
(Plasmid #200883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200883 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC28-Bsa4
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HiBIT-WDR5
  • Species
    H. sapiens (human)
  • Mutation
    WT
  • Entrez Gene
    WDR5 (a.k.a. BIG-3, BIG3, CFAP89, SWD3)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNIC28-HiBIT-WDR5 was a gift from Stefan Knapp (Addgene plasmid # 200883 ; http://n2t.net/addgene:200883 ; RRID:Addgene_200883)

  • For your References section:

    Tracking the PROTAC degradation pathway in living cells highlights the importance of ternary complex measurement for PROTAC optimization. Schwalm MP, Kramer A, Dolle A, Weckesser J, Yu X, Jin J, Saxena K, Knapp S. Cell Chem Biol. 2023 Jun 20:S2451-9456(23)00157-5. doi: 10.1016/j.chembiol.2023.06.002. 10.1016/j.chembiol.2023.06.002 PubMed 37354907
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