pF-N-HiBiT-template
(Plasmid
#200882)
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Purposetemplate for N-terminal HiBIT-tagging
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200882 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepF-31Kp
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHiBIT-linker
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Speciesother
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MutationWT
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.01.11.523589v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pF-N-HiBiT-template was a gift from Stefan Knapp (Addgene plasmid # 200882 ; http://n2t.net/addgene:200882 ; RRID:Addgene_200882) -
For your References section:
Tracking the PROTAC degradation pathway in living cells highlights the importance of ternary complex measurement for PROTAC optimization. Schwalm MP, Kramer A, Dolle A, Weckesser J, Yu X, Jin J, Saxena K, Knapp S. Cell Chem Biol. 2023 Jun 20:S2451-9456(23)00157-5. doi: 10.1016/j.chembiol.2023.06.002. 10.1016/j.chembiol.2023.06.002 PubMed 37354907
