pEF 1-PHD-4
(Plasmid
#20088)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20088 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR221-P4P1R
- Backbone size w/o insert (bp) 4777
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Vector typeSynthetic Biology ; plant Gateway vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePHD
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SpeciesS. lycopersicum
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Insert Size (bp)1499
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GenBank IDFJ586232
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositing scientist is aware of discrepancies between Addgene's sequence and their own. They have confirmed the differences and tested the plasmids--they are completely functional.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF 1-PHD-4 was a gift from Antonio Richart (Addgene plasmid # 20088 ; http://n2t.net/addgene:20088 ; RRID:Addgene_20088)
