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Addgene

GID4-Nluc
(Plasmid #200879)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200879 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pF-32Kp
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GID4-NanoLuciferase
  • Species
    H. sapiens (human)
  • Mutation
    WT
  • Entrez Gene
    C17orf39 (a.k.a. MGC3048)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GID4-Nluc was a gift from Stefan Knapp (Addgene plasmid # 200879 ; http://n2t.net/addgene:200879 ; RRID:Addgene_200879)

  • For your References section:

    A chemical probe to modulate human GID4 Pro/N-degron interactions. Owens DGD, Maitland E.R, Yazdi AK, Song X, Schwalm MP, Machado RAC, Bauer N , Wang X, Szewczyk MM, Dong C , Dong A, Loppnau P, Calabrese MF, Dowling MS, Lee J, Montgomery JI, O'Connell TN, Subramanyam C, Wang F, Schapira M, Knapp S , Vedadi M, Min J, Lajoie GA, Barsyte-Lovejoy D , Owen DR, Schild-Poulter C, Arrowsmith CH. bioRxiv 2023.01.17.524225 10.1101/2023.01.17.524225
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