NLuc-GID4
(Plasmid
#200878)
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PurposeMammalian expression of human GID4 with N-terminal NanoLuciferase fusion
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200878 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepF-31Kp
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNanoLuciferase-GID4
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SpeciesH. sapiens (human)
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MutationWT
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Entrez GeneC17orf39 (a.k.a. MGC3048)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.01.17.524225v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NLuc-GID4 was a gift from Stefan Knapp (Addgene plasmid # 200878 ; http://n2t.net/addgene:200878 ; RRID:Addgene_200878) -
For your References section:
A chemical probe to modulate human GID4 Pro/N-degron interactions. Owens DGD, Maitland E.R, Yazdi AK, Song X, Schwalm MP, Machado RAC, Bauer N , Wang X, Szewczyk MM, Dong C , Dong A, Loppnau P, Calabrese MF, Dowling MS, Lee J, Montgomery JI, O'Connell TN, Subramanyam C, Wang F, Schapira M, Knapp S , Vedadi M, Min J, Lajoie GA, Barsyte-Lovejoy D , Owen DR, Schild-Poulter C, Arrowsmith CH. bioRxiv 2023.01.17.524225 10.1101/2023.01.17.524225
