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Addgene

pSCT376: pTwinStrep-bdSUMO-ZooTsy_TnsF-Y584F
(Plasmid #200877)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200877 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    ColE1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ZooTsy_TnsF
  • Species
    Zoogloea sp. LCSB751
  • Mutation
    TnsF-Y584F

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSCT376: pTwinStrep-bdSUMO-ZooTsy_TnsF-Y584F was a gift from Feng Zhang (Addgene plasmid # 200877 ; http://n2t.net/addgene:200877 ; RRID:Addgene_200877)
  • For your References section:

    Modularity and diversity of target selectors in Tn7 transposons. Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F. Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1. 10.1016/j.molcel.2023.05.013 PubMed 37267947