pSCT162: pTwinStrep-bdSUMO-AjTn6022_TnsF
(Plasmid
#200865)
-
PurposeAjTn6022_TniQ expression construct for protein purification.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 200865 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneColE1
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameAjTn6022_TnsF
-
SpeciesAcinetobacter johnsonii
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSCT162: pTwinStrep-bdSUMO-AjTn6022_TnsF was a gift from Feng Zhang (Addgene plasmid # 200865 ; http://n2t.net/addgene:200865 ; RRID:Addgene_200865) -
For your References section:
Modularity and diversity of target selectors in Tn7 transposons. Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F. Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1. 10.1016/j.molcel.2023.05.013 PubMed 37267947