pMSIP116: pHelper(CyCAST)_PSP1
(Plasmid
#200840)
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PurposeInducible expression of CyCAST proteins with crRNA targeting CyPSP1.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200840 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDFDuet-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCyCAST
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SpeciesCyanothece sp. PCC 7425
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSIP116: pHelper(CyCAST)_PSP1 was a gift from Feng Zhang (Addgene plasmid # 200840 ; http://n2t.net/addgene:200840 ; RRID:Addgene_200840) -
For your References section:
Modularity and diversity of target selectors in Tn7 transposons. Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F. Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1. 10.1016/j.molcel.2023.05.013 PubMed 37267947