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Addgene

pLenti4-Puro-H19
(Plasmid #200835)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200835 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLenti4-Puro (Addgene plasmid #39481)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6996
  • Total vector size (bp) 9284
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human long non-coding RNA H19
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2288
  • GenBank ID
    ENST00000412788
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti4-Puro-H19 was a gift from Reinier Boon (Addgene plasmid # 200835 ; http://n2t.net/addgene:200835 ; RRID:Addgene_200835)
  • For your References section:

    Long non-coding RNA H19 regulates endothelial cell aging via inhibition of STAT3 signalling. Hofmann P, Sommer J, Theodorou K, Kirchhof L, Fischer A, Li Y, Perisic L, Hedin U, Maegdefessel L, Dimmeler S, Boon RA. Cardiovasc Res. 2019 Jan 1;115(1):230-242. doi: 10.1093/cvr/cvy206. 10.1093/cvr/cvy206 PubMed 30107531