pLenti4-Puro-H19
(Plasmid
#200835)
-
PurposeLentiviral vector expressing human long non-coding RNA H19
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 200835 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepLenti4-Puro (Addgene plasmid #39481)
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 6996
- Total vector size (bp) 9284
-
Vector typeLentiviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHuman long non-coding RNA H19
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2288
-
GenBank IDENST00000412788
- Promoter CMV
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer V5 (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLenti4-Puro-H19 was a gift from Reinier Boon (Addgene plasmid # 200835 ; http://n2t.net/addgene:200835 ; RRID:Addgene_200835) -
For your References section:
Long non-coding RNA H19 regulates endothelial cell aging via inhibition of STAT3 signalling. Hofmann P, Sommer J, Theodorou K, Kirchhof L, Fischer A, Li Y, Perisic L, Hedin U, Maegdefessel L, Dimmeler S, Boon RA. Cardiovasc Res. 2019 Jan 1;115(1):230-242. doi: 10.1093/cvr/cvy206. 10.1093/cvr/cvy206 PubMed 30107531