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Addgene

pcDNA3/UBC9
(Plasmid #20082)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20082 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UBC9
  • Species
    H. sapiens (human)
  • Entrez Gene
    UBE2I (a.k.a. C358B7.1, P18, UBC9)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3/UBC9 was a gift from Edward Yeh (Addgene plasmid # 20082 ; http://n2t.net/addgene:20082 ; RRID:Addgene_20082)

  • For your References section:

    Preferential interaction of sentrin with a ubiquitin-conjugating enzyme, Ubc9. Gong L, Kamitani T, Fujise K, Caskey LS, Yeh ET. J Biol Chem. 1997 Nov 7;272(45):28198-201. PubMed 9353268
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