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Addgene

pCAGGS-FLAG-BubR1-D911A
(Plasmid #200815)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200815 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4841
  • Total vector size (bp) 7991
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BUB1 mitotic checkpoint serine/threonine kinase B
  • Alt name
    MAD3L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3150
  • Mutation
    Change Aspartic acid 911 to Alanine
  • GenBank ID
    NM_001211.6
  • Entrez Gene
    BUB1B (a.k.a. BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1)
  • Promoter CAG
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer GTTCGGCTTCTGGCGTGTGAC
  • 3′ sequencing primer TATGTCCTTCCGAGTGAGAGAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-FLAG-BubR1-D911A was a gift from Hung-Chun Chang (Addgene plasmid # 200815 ; http://n2t.net/addgene:200815 ; RRID:Addgene_200815)
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