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pJH4677
(Plasmid #200782)

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Item Catalog # Description Quantity Price (USD)
Plasmid 200782 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pBSK
  • Backbone manufacturer
    stratagene
  • Backbone size w/o insert (bp) 8204
  • Total vector size (bp) 8495
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ins-22
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    291
  • Entrez Gene
    ins-22 (a.k.a. CELE_M04D8.2)
  • Promoter Pnpr-4
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tgctgtcataatacgggagtacCCGGGatgcacactacaactattctcat
  • 3′ sequencing primer CTCGACCAGGATGGGCACCACCCCGGTGAACagctcctcgcccttcaccataggtacc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH4677 was a gift from Mei Zhen (Addgene plasmid # 200782 ; http://n2t.net/addgene:200782 ; RRID:Addgene_200782)

  • For your References section:

    A tonically active master neuron modulates mutually exclusive motor states at two timescales. Meng J, Ahamed T, Yu B, Hung W, Ei Mouridi S, Wang Z, Zhang Y, Wen Q, Boulin T, Gao S, Zhen M. Sci Adv. 2024 Apr 12;10(15):eadk0002. doi: 10.1126/sciadv.adk0002. Epub 2024 Apr 10. 10.1126/sciadv.adk0002 PubMed 38598630
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