AAV-HAPLN1
(Plasmid
#200777)
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PurposeExpresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200777 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-Syn_MCS_WPRE_Sv40pA
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Backbone manufacturerVectorBuilder
- Backbone size w/o insert (bp) 4949
- Total vector size (bp) 6647
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHyaluronan And Proteoglycan Link Protein 1
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Alt nameHAPLN1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1068
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Entrez GeneHapln1 (a.k.a. CLP, Crtl1, Crtl1l, LP, LP-1)
- Promoter Synapsin
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Tag
/ Fusion Protein
- cfGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer GCCGCCTCAGCACTGAAG
- 3′ sequencing primer ACTTGTGGCCGTTTACGTC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-HAPLN1 was a gift from Alexander Dityatev (Addgene plasmid # 200777 ; http://n2t.net/addgene:200777 ; RRID:Addgene_200777) -
For your References section:
A shift in the mechanisms controlling hippocampal engram formation during brain maturation. Ramsaran AI, Wang Y, Golbabaei A, Aleshin S, de Snoo ML, Yeung BA, Rashid AJ, Awasthi A, Lau J, Tran LM, Ko SY, Abegg A, Duan LC, McKenzie C, Gallucci J, Ahmed M, Kaushik R, Dityatev A, Josselyn SA, Frankland PW. Science. 2023 May 5;380(6644):543-551. doi: 10.1126/science.ade6530. Epub 2023 May 4. 10.1126/science.ade6530 PubMed 37141366