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Addgene

pH-ABE8e-spG
(Plasmid #200775)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200775 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHUE411 (modified from pCambia)
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nCas9-spG(D10A)-TadA8e
  • Alt name
    TadA8e-32aa linker-nCas9-spG(D10A)-3NLS
  • Species
    Streptococcus pyogenes for Cas9; E. coli for TadA
  • Mutation
    D10A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R for Cas9; W23R, H36L, P48A, R51L, L84F, A106V, D108N, A109S, T111R, D11l9N, H122N, H123Y, S146C, F149Y, R152P, E155V, I156F, K157N, T166I, D167N for TadA
  • Promoter maize Ubiquitin-1, OsU3

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pH-ABE8e-spG was a gift from Caixia Gao (Addgene plasmid # 200775 ; http://n2t.net/addgene:200775 ; RRID:Addgene_200775)

  • For your References section:

    Tuning plant phenotypes by precise, graded downregulation of gene expression. Xue C, Qiu F, Wang Y, Li B, Zhao KT, Chen K, Gao C. Nat Biotechnol. 2023 Mar 9. doi: 10.1038/s41587-023-01707-w. 10.1038/s41587-023-01707-w PubMed 36894598
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