RS314-U6(PolII-Lsm)
(Plasmid #200772)

• Purpose: Pol II expression of U6 Lsm
• Depositing Lab: Aaron Hoskins
• Publication: Lipinski et al RNA. 2022 Dec;28(12):1606-1620. doi: 10.1261/rna.079328.122. Epub 2022 Oct 4.

Backbone

• Vector backbone: pRS314
• Backbone manufacturer: ATCC
• Backbone size w/o insert (bp): 4783
• Total vector size (bp): 6045
• Modifications to backbone: The WT U6 is driven by a GAL1 promoter, U6 coding sequence is inserted between SNR14(-100/-1) and SNR14(+296/+701), in the pRS314 vector
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Gal-SNR6-II
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1262

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CCGCTCGAGATCATATTACATGGCATTACCA
• 3′ sequencing primer: CGGGGTACCTTCCTCTCTGCTGTTTTAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry