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Addgene

pMSCV-Flag-cMyc T58A
(Plasmid #20075)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20075 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5111
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cMyc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Mutation
    T58A
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Puro resistance cassette was removed by cutting pMSCVpuro with EcoRI and ClaI and religating. EcoRI site was maintained in the vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-Flag-cMyc T58A was a gift from Juan Belmonte (Addgene plasmid # 20075 ; http://n2t.net/addgene:20075 ; RRID:Addgene_20075)

  • For your References section:

    Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, Vassena R, Bilić J, Pekarik V, Tiscornia G, Edel M, Boué S, Belmonte JC. Nat Biotechnol. 2008 Nov . 26(11):1276-84. 10.1038/nbt.1503 PubMed 18931654
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