Skip to main content
Addgene

GFP-Atg32-deltaPsR pXP721
(Plasmid #200735)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200735 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pXP721
  • Total vector size (bp) 7124
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Atg32
  • Alt name
    ECM37
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Amino acids 200-341 are deleted.
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-Atg32-deltaPsR pXP721 was a gift from Michael Ragusa (Addgene plasmid # 200735 ; http://n2t.net/addgene:200735 ; RRID:Addgene_200735)

  • For your References section:

    A pseudo-receiver domain in Atg32 is required for mitophagy. Xia X, Katzenell S, Reinhart EF, Bauer KM, Pellegrini M, Ragusa MJ. Autophagy. 2018;14(9):1620-1628. doi: 10.1080/15548627.2018.1472838. Epub 2018 Aug 1. 10.1080/15548627.2018.1472838 PubMed 29909755
Related items: