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Addgene

pLKO.1 LARP7-FLAG m6A mut 7SK
(Plasmid #200734)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200734 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LARP7 and 7SK
  • Species
    H. sapiens (human)
  • Mutation
    7SK m6A mutations: A172G, A220T, A228G, A281G, and A288G
  • Entrez Gene
    LARP7 (a.k.a. ALAZS, HDCMA18P, PIP7S, hLARP7)
  • Promoter U6
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1 LARP7-FLAG m6A mut 7SK was a gift from Claudio Alarcón (Addgene plasmid # 200734 ; http://n2t.net/addgene:200734 ; RRID:Addgene_200734)
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