pLKO.1 LARP7-FLAG 7SK
(Plasmid
#200733)
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PurposeLentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200733 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLKO.1
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLARP7 and 7SK
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SpeciesH. sapiens (human)
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Entrez GeneLARP7 (a.k.a. ALAZS, HDCMA18P, PIP7S, hLARP7)
- Promoter U6
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLKO.1 LARP7-FLAG 7SK was a gift from Claudio Alarcón (Addgene plasmid # 200733 ; http://n2t.net/addgene:200733 ; RRID:Addgene_200733)