pLenti-C-Myc-DDK-IRES-Puro HNRNPA1
(Plasmid
#200727)
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PurposeLentiviral expression of Flag-Tagged human HNRNPA1
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200727 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti-C-Myc-DDK-IRES-Puro
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Backbone manufacturerOrigene
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHNRNPA1
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SpeciesH. sapiens (human)
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Entrez GeneHNRNPA1 (a.k.a. ALS19, ALS20, HNRPA1, HNRPA1L3, IBMPFD3, MPD3, UP 1, hnRNP A1, hnRNP-A1)
- Promoter CMV
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Tag
/ Fusion Protein
- Myc-DDK from backbone plasmid (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer V2-F
- 3′ sequencing primer IRES100-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-C-Myc-DDK-IRES-Puro HNRNPA1 was a gift from Claudio Alarcón (Addgene plasmid # 200727 ; http://n2t.net/addgene:200727 ; RRID:Addgene_200727)