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Addgene

pLenti-C-Myc-DDK-IRES-Puro METTL3
(Plasmid #200724)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200724 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-C-Myc-DDK-IRES-Puro
  • Backbone manufacturer
    Origene
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    METTL3
  • Species
    H. sapiens (human)
  • Entrez Gene
    METTL3 (a.k.a. IME4, M6A, MT-A70, Spo8, hMETTL3)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc-DDK from backbone plasmid (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer V2-F
  • 3′ sequencing primer IRES100-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-C-Myc-DDK-IRES-Puro METTL3 was a gift from Claudio Alarcón (Addgene plasmid # 200724 ; http://n2t.net/addgene:200724 ; RRID:Addgene_200724)
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