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Addgene

TS181_pETNKI_his3C_pA-Dam
(Plasmid #200721)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200721 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET NKI
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    His3C-pA-Dam
  • Alt name
    ECO-Dam methylase
  • Species
    bacteria
  • Mutation
    codon optimized
  • Tag / Fusion Protein
    • His (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TS181_pETNKI_his3C_pA-Dam was a gift from Bas van Steensel (Addgene plasmid # 200721 ; http://n2t.net/addgene:200721 ; RRID:Addgene_200721)

  • For your References section:

    Genome-Wide Mapping and Microscopy Visualization of Protein-DNA Interactions by pA-DamID. van Schaik T, Manzo SG, van Steensel B. Methods Mol Biol. 2022;2458:215-229. doi: 10.1007/978-1-0716-2140-0_12. 10.1007/978-1-0716-2140-0_12 PubMed 35103970
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