TS246_pNKI-2-13_HALO-DPN7
(Plasmid
#200720)
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Purposeproducing Methyl Tracer HALO protein in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200720 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNKI
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHALO-DPN7
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Alt nameM6A tracer
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Speciesbacteria
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Mutationcodon optimized
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TS246_pNKI-2-13_HALO-DPN7 was a gift from Bas van Steensel (Addgene plasmid # 200720 ; http://n2t.net/addgene:200720 ; RRID:Addgene_200720) -
For your References section:
Genome-Wide Mapping and Microscopy Visualization of Protein-DNA Interactions by pA-DamID. van Schaik T, Manzo SG, van Steensel B. Methods Mol Biol. 2022;2458:215-229. doi: 10.1007/978-1-0716-2140-0_12. 10.1007/978-1-0716-2140-0_12 PubMed 35103970