pMSP1E3D1
(Plasmid #20066)

• Purpose: Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation
• Depositing Lab: Stephen Sligar
• Publication: Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76.

Backbone

• Vector backbone: pET 28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL-21(DE3); high oxygenation level
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MSP1E3D1
• Alt name: membrane scaffold protein
• Species: synthetic gene
• Insert Size (bp): 840
• Mutation: "extended" MSP1D1; contains repeats of helices 4, 5 and 6;N-terminal 7-his tag followed by spacer sequence and TEV protease cleavage site
• Tag / Fusion Protein:
  • 7-His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nco I (not destroyed)
• 3′ cloning site: Hind III (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Resource Information

• Articles Citing this Plasmid:
  • 55 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry