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pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA
(Plasmid #200641)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200641 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6662
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miniTurboID-6xGGSG linker-FKBPF36V-2xHA
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1269
  • Promoter T7
  • Tag / Fusion Protein
    • HA-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.08.21.554211 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA was a gift from Justin English & Fleur Ferguson (Addgene plasmid # 200641 ; http://n2t.net/addgene:200641 ; RRID:Addgene_200641)

  • For your References section:

    A biotin targeting chimera (BioTAC) system to map small molecule interactomes in situ. Tao AJ, Jiang J, Gadbois GE, Goyal P, Boyle BT, Mumby EJ, Myers SA, English JG, Ferguson FM. Nat Commun. 2023 Dec 4;14(1):8016. doi: 10.1038/s41467-023-43507-5. 10.1038/s41467-023-43507-5 PubMed 38049406
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