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Addgene

1114H
(Plasmid #200640)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200640 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBac
  • Backbone manufacturer
    unknown
  • Modifications to backbone
    PiggyBac expression casette added
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
  • gRNA/shRNA sequence
    GATCCGATCACGCAGTCGAT, GCTCGATATCGTGCGCAAGG, CCAAATAGGCGCTAAGTTCT, TTATCATCCACTCTGACGGG, GTTTAACACAGGTCAAGCGG
  • Species
    An. gambiae
  • GenBank ID
    AGAP006241

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGTAAAACGACGGCCAGT
  • 3′ sequencing primer CTTGGAGCTCCCGTGAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1114H was a gift from Omar Akbari (Addgene plasmid # 200640 ; http://n2t.net/addgene:200640 ; RRID:Addgene_200640)

  • For your References section:

    Eliminating Malaria Vectors with Precision Guided Sterile Males. Smidler AL, Apte RA, Pai JJ, Chow ML, Chen S, Mondal A, Sanchez C HM, Antoshechkin I, Marshall JM, Akbari OS. bioRxiv [Preprint]. 2023 Jul 21:2023.07.20.549947. doi: 10.1101/2023.07.20.549947. 10.1101/2023.07.20.549947 PubMed 37503146
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