-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 20064 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepET 28a
-
Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5300
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsBL-21(DE3); high oxygenation level
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameMSP1E3
-
Alt namemembrane scaffold protein
-
Alt nameAPOA1
-
Speciessynthetic gene
-
Insert Size (bp)870
-
Mutation"extended" MSP1; contains repeats of helices 4, 5 and 6
-
Entrez GeneAPOA1 (a.k.a. AMYLD3, HPALP2, apo(a))
-
Tag
/ Fusion Protein
- 6-His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nco I (not destroyed)
- 3′ cloning site Hind III (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMSP1E3 was a gift from Stephen Sligar (Addgene plasmid # 20064 ; http://n2t.net/addgene:20064 ; RRID:Addgene_20064) -
For your References section:
Directed self-assembly of monodisperse phospholipid bilayer Nanodiscs with controlled size. Denisov IG, Grinkova YV, Lazarides AA, Sligar SG. J Am Chem Soc. 2004 Mar 24. 126(11):3477-87. 10.1021/ja0393574 PubMed 15025475