G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3
(Plasmid
#200631)
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PurposeNegative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GA) landing pad
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200631 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLenti
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Vector typeLentiviral
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Selectable markersBlasticidin ; mTagBFP2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameiCasp9
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SpeciesSynthetic
- Promoter pTRE3G
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3 was a gift from Kenneth Matreyek (Addgene plasmid # 200631 ; http://n2t.net/addgene:200631 ; RRID:Addgene_200631) -
For your References section:
Mammalian Genomic Manipulation with Orthogonal Bxb1 DNA Recombinase Sites for the Functional Characterization of Protein Variants. Roelle SM, Kamath ND, Matreyek KA. ACS Synth Biol. 2023 Nov 3. doi: 10.1021/acssynbio.3c00355. 10.1021/acssynbio.3c00355 PubMed 37922210
