pVS45
(Plasmid
#20059)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20059 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC13
- Backbone size w/o insert (bp) 2658
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerat hepatic cytochrome b5
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SpeciesR. norvegicus (rat)
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Insert Size (bp)334
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Pst I (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVS45 was a gift from Stephen Sligar (Addgene plasmid # 20059 ; http://n2t.net/addgene:20059 ; RRID:Addgene_20059) -
For your References section:
Synthesis, bacterial expression, and mutagenesis of the gene coding for mammalian cytochrome b5. Beck von Bodman S, Schuler MA, Jollie DR, Sligar SG. Proc Natl Acad Sci U S A. 1986 Dec . 83(24):9443-7. 10.1073/pnas.83.24.9443 PubMed 3540940