pDEST40-CES2C-ΔC
(Plasmid
#200555)
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PurposeMammalian expression of secreted mouse CES2C
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200555 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST40
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMouse Carboxylesterase 2C delta C-terminal
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SpeciesM. musculus (mouse)
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Entrez GeneCes2c (a.k.a. ACH M1, CES 2, Ces2, ces2A3)
- Promoter CMV promoter
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Tags
/ Fusion Proteins
- Flag (N terminal on insert)
- V5 (C terminal on insert)
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 forward
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST40-CES2C-ΔC was a gift from Jonathan Long (Addgene plasmid # 200555 ; http://n2t.net/addgene:200555 ; RRID:Addgene_200555) -
For your References section:
Organism-wide, cell-type-specific secretome mapping of exercise training in mice. Wei W, Riley NM, Lyu X, Shen X, Guo J, Raun SH, Zhao M, Moya-Garzon MD, Basu H, Sheng-Hwa Tung A, Li VL, Huang W, Wiggenhorn AL, Svensson KJ, Snyder MP, Bertozzi CR, Long JZ. Cell Metab. 2023 Apr 28:S1550-4131(23)00138-9. doi: 10.1016/j.cmet.2023.04.011. 10.1016/j.cmet.2023.04.011 PubMed 37141889