pSB305-K3L-vac-HA
(Plasmid
#20054)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20054 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSB305
- Backbone size w/o insert (bp) 5500
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameK3L
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SpeciesVaccinia virus (copenhagen strain)
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Insert Size (bp)297
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSB305-K3L-vac-HA was a gift from Harmit Malik (Addgene plasmid # 20054 ; http://n2t.net/addgene:20054 ; RRID:Addgene_20054) -
For your References section:
Protein kinase R reveals an evolutionary model for defeating viral mimicry. Elde NC, Child SJ, Geballe AP, Malik HS. Nature. 2008 Nov 30. ():. 10.1038/nature07529 PubMed 19043403