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Addgene

pSB305-K3L-vac
(Plasmid #20053)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20053 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB305
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    K3L
  • Species
    Vaccinia virus (copenhagen strain)
  • Insert Size (bp)
    267

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer M13F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB305-K3L-vac was a gift from Harmit Malik (Addgene plasmid # 20053 ; http://n2t.net/addgene:20053 ; RRID:Addgene_20053)

  • For your References section:

    Protein kinase R reveals an evolutionary model for defeating viral mimicry. Elde NC, Child SJ, Geballe AP, Malik HS. Nature. 2008 Nov 30. ():. 10.1038/nature07529 PubMed 19043403
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