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pAWO-SUMO-3C_14M_zTDP2
(Plasmid #200512)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200512 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-17b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TDP2
  • Species
    D. rerio (zebrafish)
  • Mutation
    L136N, A139S, C239T, K240R, K309Q, K314L, T315G, V316I, P317T, Y318A, V319A, S320C, R321K, C322L
  • Entrez Gene
    tdp2b (a.k.a. tdp2, ttrap, ttrapl)
  • Tag / Fusion Protein
    • HIS-SUMO (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site EcoRi (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The gene was made synthetically (i.e. bought from a company)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAWO-SUMO-3C_14M_zTDP2 was a gift from Antony Oliver (Addgene plasmid # 200512 ; http://n2t.net/addgene:200512 ; RRID:Addgene_200512)
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