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pSB819-PKR-hum-LYA
(Plasmid #20050)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20050 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PSB819
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Protein kinase R
  • Alt name
    EIF2AK2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1656
  • Mutation
    F489Y, S492A
  • GenBank ID
    NM_002759
  • Entrez Gene
    EIF2AK2 (a.k.a. DYT33, EIF2AK1, LEUDEN, PKR, PPP1R83, PRKR)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB819-PKR-hum-LYA was a gift from Harmit Malik (Addgene plasmid # 20050 ; http://n2t.net/addgene:20050 ; RRID:Addgene_20050)

  • For your References section:

    Protein kinase R reveals an evolutionary model for defeating viral mimicry. Elde NC, Child SJ, Geballe AP, Malik HS. Nature. 2008 Nov 30. ():. 10.1038/nature07529 PubMed 19043403
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