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Addgene

pcDNA3.1-(-)-Pur
(Plasmid #200458)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200458 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Thermofisher
  • Backbone size (bp) 5185
  • Modifications to backbone
    The initial vector was pcDNA3.1-(-)-Hyg. The Hygromycin ORF was replace with the Puromycin ORF amplified from Promega pGL4.22-Pur. The final vector was obtained by PCR fragments assembly.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Original backbone from Thermofisher (pcDNA3.1-(-)-Hyg, insert amplified from Promega pGL4.22-Pur

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-(-)-Pur was a gift from Bertrand Collet (Addgene plasmid # 200458 ; http://n2t.net/addgene:200458 ; RRID:Addgene_200458)
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