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Addgene

3xFlag-CEP83
(Plasmid #200442)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200442 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+N-3xFlag Cloning Vector (#196651)
  • Backbone size w/o insert (bp) 4160
  • Total vector size (bp) 6217
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CEP83
  • Alt name
    CCDC41
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2079
  • Entrez Gene
    CCDC41 (a.k.a. MGC149726, NY-REN-58)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFlag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GCGTGCCTAATGGGAGGTCT (CSF)
  • 3′ sequencing primer CACTGCATTCTAGTTCTGGT (CSR)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3xFlag-CEP83 was a gift from Ken-Ichi Takemaru (Addgene plasmid # 200442 ; http://n2t.net/addgene:200442 ; RRID:Addgene_200442)
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