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Addgene

Atg20-24 pST39
(Plasmid #200439)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200439 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pST39
  • Total vector size (bp) 60093
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Atg20
  • Alt name
    CVT20
  • Alt name
    SNX42
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1977
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    Atg24
  • Alt name
    SNX4
  • Alt name
    CVT13
  • Insert Size (bp)
    1275

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Atg20-24 pST39 was a gift from Michael Ragusa (Addgene plasmid # 200439 ; http://n2t.net/addgene:200439 ; RRID:Addgene_200439)

  • For your References section:

    A Comparative Analysis of the Membrane Binding and Remodeling Properties of Two Related Sorting Nexin Complexes Involved in Autophagy. Reinhart EF, Katzenell S, Andhare D, Bauer KM, Ragusa MJ. Biochemistry. 2022 Apr 14. doi: 10.1021/acs.biochem.2c00007. 10.1021/acs.biochem.2c00007 PubMed 35421303
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