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Addgene

PC1-29-9-2_GAL4-VHL
(Plasmid #200434)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200434 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Custom
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GAL4-VHL
  • Species
    Synthetic
  • Insert Size (bp)
    1108
  • Tag / Fusion Protein
    • GAL4, VHL

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGGCAACGTGCTGGTTATTGTGC
  • 3′ sequencing primer CTCCTCAGGTGCAGGCTGCCTA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PC1-29-9-2_GAL4-VHL was a gift from Xue Gao (Addgene plasmid # 200434 ; http://n2t.net/addgene:200434 ; RRID:Addgene_200434)

  • For your References section:

    Engineered PROTAC-CID Systems for Mammalian Inducible Gene Regulation. Ma D, Yuan Q, Peng F, Paredes V, Zeng H, Osikpa EC, Yang Q, Peddi A, Patel A, Liu MS, Sun Z, Gao X. J Am Chem Soc. 2023 Jan 25;145(3):1593-1606. doi: 10.1021/jacs.2c09129. Epub 2023 Jan 10. 10.1021/jacs.2c09129 PubMed 36626587
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