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CMV EGFP-PPM1H-H153D
(Plasmid #200432)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200432 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5D GFPP:PPM1H WT
  • Backbone manufacturer
    MRC PPU #DU62939
  • Backbone size w/o insert (bp) 4300
  • Total vector size (bp) 5800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPM1H protein phosphatase, Mg2+/Mn2+ dependent 1H
  • Alt name
    URCC2
  • Alt name
    ARHCL1
  • Alt name
    NERPP-2C
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    15000
  • Mutation
    H153D
  • GenBank ID
    NM_020700.1
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer 5'-GACCACTACCAGCAGAACAC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    MRC PPU #DU66215

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV EGFP-PPM1H-H153D was a gift from Erika Holzbaur (Addgene plasmid # 200432 ; http://n2t.net/addgene:200432 ; RRID:Addgene_200432)

  • For your References section:

    Regulatory imbalance between LRRK2 kinase, PPM1H phosphatase, and ARF6 GTPase disrupts the axonal transport of autophagosomes. Dou D, Smith EM, Evans CS, Boecker CA, Holzbaur ELF. Cell Rep. 2023 May 1;42(5):112448. doi: 10.1016/j.celrep.2023.112448. 10.1016/j.celrep.2023.112448 PubMed 37133994
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