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Addgene

PGK 4xMito-mEmerald
(Plasmid #200430)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200430 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1/PGK
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3900
  • Total vector size (bp) 5100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4x cytochrome c oxidase subunit VIII mitochondrial targeting sequence
  • Species
    Synthetic
  • Insert Size (bp)
    1200
  • Mutation
    4x mito-targeting sequence
  • Promoter PGK
  • Tag / Fusion Protein
    • mEmerald (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NarI (unknown if destroyed)
  • 3′ cloning site BsrGI (unknown if destroyed)
  • 5′ sequencing primer 5'-TGGGGTTGCGCCTTTTCC
  • 3′ sequencing primer 5'-CTTTATTTGTAACCATTATAAGCTGC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene #98818

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PGK 4xMito-mEmerald was a gift from Erika Holzbaur (Addgene plasmid # 200430 ; http://n2t.net/addgene:200430 ; RRID:Addgene_200430)

  • For your References section:

    Regulatory imbalance between LRRK2 kinase, PPM1H phosphatase, and ARF6 GTPase disrupts the axonal transport of autophagosomes. Dou D, Smith EM, Evans CS, Boecker CA, Holzbaur ELF. Cell Rep. 2023 May 1;42(5):112448. doi: 10.1016/j.celrep.2023.112448. 10.1016/j.celrep.2023.112448 PubMed 37133994
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