pIA033
(Plasmid #200420)

• Purpose: (Empty Backbone) thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.
• Depositing Lab: Rut Carballido-López
• Publication: Altinoglu et al bioRxiv 2023

Backbone

• Vector backbone: pDG1664
• Backbone size (bp): 6102
• Vector type: Bacterial Expression
• Selectable markers: Erythromycin
• Tag / Fusion Protein:
  • bsPAmCherry (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: E. coli: 37°C and selection ampicillin 100 μg/mL B. subtilis: 30°C and selection erythromycin 0.5 μg/mL
• Copy number: Low Copy

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pIA033 aka ThrC-x-bsPAmCherry.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

bsPAmCherry1 was amplified from pIA020 using primers oIA056 and oIA057, then cloned into pIA023 digested with BamHI and HindIII, giving plasmid pIA033, thrC::(bsPamCherry1 erm2).