pIA028
(Plasmid #200419)

• Purpose: (Empty Backbone) thrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloning
• Depositing Lab: Rut Carballido-López
• Publication: Altinoglu et al bioRxiv 2023

Backbone

• Vector backbone: pDG1664
• Backbone size (bp): 6102
• Vector type: Bacterial Expression
• Selectable markers: Erythromycin
• Tag / Fusion Protein:
  • PAmCherry1 (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: E. coli: 37°C and selection ampicillin 100 μg/mL B. subtilis: 30°C and selection erythromycin 0.5 μg/mL
• Copy number: Low Copy

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pIA028 aka ThrC-[x]-PAmCherry (Gibson).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The PAmCherry1 sequence was amplified from pEYY133 using primers oIA008 and oIA048, then cloned into pIA023 digested with BamHI and SphI, resulting in plasmid pIA028.