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Addgene

pIA027
(Plasmid #200418)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200418 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDG364
  • Backbone size (bp) 6310
  • Vector type
    Bacterial Expression
  • Selectable markers
    Chloramphenicol
  • Tag / Fusion Protein
    • dronPA (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E. coli: 37°C and selection Ampicillin 100 μg/mL B. subtilis: 30°C and selection Chloramphenicol 5 μg/mL
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The dronPA sequence was amplified from pEYY99 using primers oIA010 and oIA047, then cloned into pIA021 digested with BamHI and SbfI, resulting in plasmid pIA027

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIA027 was a gift from Rut Carballido-López (Addgene plasmid # 200418 ; http://n2t.net/addgene:200418 ; RRID:Addgene_200418)

  • For your References section:

    New PALM-compatible integration vectors for use in the Gram-positive model bacterium Bacillus subtilis. Altinoglu I, Carballido-Lopez R. bioRxiv 2023 10.1101/2023.03.16.532899
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