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Addgene

pOPARA1
(Plasmid #200416)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200416 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD24
  • Modifications to backbone
    Addition of BsaI restriction sites flanking an RFP negative selection cassette (for Golden Gate cloning); Addition of Esp3I restriction sites flanking carbenicillin resistance cassette; Domestication of BsaI and Esp3I sites.
  • Vector type
    Bacterial Expression
  • Promoter pBAD

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPARA1 was a gift from Kamil Witek (Addgene plasmid # 200416 ; http://n2t.net/addgene:200416 ; RRID:Addgene_200416)

  • For your References section:

    pOPARA: Vectors for Golden Gate assembly of expression constructs containing the araBAD promoter. Lesley Milnes, Mark Youles, Josephine H. R. Maidment. bioRxiv 10.1101/2023.05.31.543011
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