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Addgene

pDV24_AAV2_DART-VADAR_iRFP720-sensor
(Plasmid #200411)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200411 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Custom
  • Vector type
    Mammalian Expression, AAV, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DART VADAR sensor
  • Species
    Synthetic
  • Promoter CMV
  • Tags / Fusion Proteins
    • TagBFP (N terminal on backbone)
    • mNeonGreen (C terminal on backbone)
    • MCP-ADAR2dd(E488Q)-NES (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Prone to recombination. This DART VADAR sensor detects the iRFP720 transcript (pDV5.x)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDV24_AAV2_DART-VADAR_iRFP720-sensor was a gift from James Collins (Addgene plasmid # 200411 ; http://n2t.net/addgene:200411 ; RRID:Addgene_200411)

  • For your References section:

    Autocatalytic base editing for RNA-responsive translational control. Gayet RV, Ilia K, Razavi S, Tippens ND, Lalwani MA, Zhang K, Chen JX, Chen JC, Vargas-Asencio J, Collins JJ. Nat Commun. 2023 Mar 11;14(1):1339. doi: 10.1038/s41467-023-36851-z. 10.1038/s41467-023-36851-z PubMed 36906659
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